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Of the three measures, the mean basal level was calculated.
The basal level was calculated as the mean of the first three time points of the nuclear-to-cytoplasmic ratio.
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Basal expression levels were calculated using the formula 2- ΔCt).
However, the mean basal insulin levels were calculated from a single sampling of each animal and not from series.
Basal miR-expression levels were calculated as 2-Δct with Δct= (ct miR-29a/29b miR-29a/29b5A)).
mRNA expression levels were calculated relative to the value obtained from CSC-cbR cells (set as basal level =1).
Basal area was calculated using Husch et al. (2003).
Plot basal area (G) was calculated by summing from the tree-level basal areas, determined as ( {scriptscriptstyle frac{pi }{4}}{D}_{bh}^2 ).
Basal metabolic rate was calculated using the FAO/WHO equation [ 29].
Fold increase in glucose uptake over basal was calculated for each individual mouse.
Basal hepatic insulin sensitivity was calculated as 100/ HGP × basal insulin secretion).
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