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Within the cell the STAT1 molecule undergoes a basal level of phosphorylation and dephosphorylation [31].
QA increased phosphorylation of serine 199/202 and threonine 231 in a dose dependent manner over the basal level of phosphorylation in the control cultures (Figure 4).
ER turnover is affected by a basal level of phosphorylation that is maintained through a balance of kinase and phosphatase activities [ 26].
These data indicate that bZIP63 has a basal level of phosphorylation in the plant and undergoes hyper-phosphorylation under starvation conditions.
We have clarified the nature of these bands (which may reflect a low basal level of phosphorylation due to MEF production of endogenous Gas6) in the legend to the revised Figure 2.
As shown in Figure 6A and B, increasing the level of TG2 in RAW264.7 macrophages decreased, while loss of TG2 in MEF cells increased the basal level of phosphorylation of JAK2, which correlates with its kinase activity.
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During the preactivation phase (3 min), basal levels of phosphorylation were further lowered by ICI, Nys, and B-TA.
We found that ZD6474 effectively suppressed basal levels of phosphorylation of S6 (S235/236), 4E-BP1 (T37/46), and Akt (S473) in a time-dependent manner in U251 and U87MG cells.
We observed that the basal levels of phosphorylation of STAT3 and Akt are reduced in VEGFR1 knockdown endothelial cells, but not in VEGFR2 knockdown cells.
As shown in Figure 2a, MAP4 showed weak basal levels of phosphorylation at S696, S768 and S787 in cultured neonatal cardiomyocytes under normoxia condition.
An intriguing finding was that although H69 and H69AR harbour a MET mutation, basal levels of phosphorylation were low in both cell lines and only when stimulated with HGF this phosphorylation increased significantly as well as the oncogenic phenotype.
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