If we apply antiperspirant in the morning, our basal level of sweat production is a bit too high, she says.
To measure basal levels of signaling in the resting, unmodulated state, the "Basal" metric was applied.
The expression level of pRB, a downstream molecule of cyclin D1, showed significant inhibition in ShCCND1 (data not shown).
Hence, we next quantified the phosphorylation level of pRB in the same lysates using reverse phase protein arrays (RPPA).
The phosphorylation level of pRB was found to be low in the growth-arrested cells and induction of pRB phosphorylation from 6 to 12 hours did not occur abruptly for CDK4 knockdown compared to MOCK.
The more likely hypothesis is the presence of a SNP in the promoter or in a regulatory element that affects the level of pRb.
Intriguingly, pRb-deficient osteoblasts also showed elevated levels of N-cadherin relative to the basal levels observed in pRb-expressing controls (Fig. 3A, middle panel), suggesting a compensation for OB-cadherin loss by a proportional increase in N-cadherin.
Total levels of pRb remained relatively unchanged, but pRb became hypophosphorylated when NET4/Tmem53 was knocked down and phosphorylated p38MAP kinase levels increased (Figure 8A,B).
Total levels of pRb appear lower, however this is likely an artifact of the antibody used, since it is known to have somewhat greater affinity for hyperphosphorylated pRb.
