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Sik1 transgene expression was detected in all clones analyzed; however, none of them reached the basal level of endogenous sik1 expression (Fig. 2B).
However, the basal level of endogenous antioxidants is similar for both the wild-type (first column) and these recombinant cells (second column) incubated with glucose.
The basal level of endogenous α-syn in SK-N-BE cell was negligible (0h lane), while TAT-α-syn 0.5 µM fusion protein was detectable from 4 h after addition to the medium, migrated as a doublet around 21KDa and even if its total amount decreased over time it was still present 48h after treatment.
We found that the basal level of endogenous ROS was significantly lower (about 16%) (P=0.001) in cyclin E-overexpressing cells compared with vector control and parental cells.
We also demonstrated that this effect likely relates to a reduced basal level of endogenous ROS and to their reduced increase following doxorubicin treatment in cyclin E-overexpressing derivatives compared with vector control cells.
Moreover, similar benefits obtained in EDL and SOL muscle indicate that SMT022357 acts independently of the fibre-type composition, the muscle structure and the basal level of endogenous utrophin.
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It is of interest to point out that the basal levels of endogenous LC3-II were higher in HeLa cells than in HEK293T cells, an observation which was in agreement with the data on eGFP-LC3 punctation (Fig. 2B).
These cells express relatively low basal levels of endogenous c-CBL (data not shown).
We routinely observe basal levels of endogenous serotonylation of proteins, and even serum-starving cells for 48 hours does not completely remove all serotonylated protein.
As a consequence, basal levels of endogenous p53, which are tightly regulated through Lys48-linked ubiquitination by MDM2, are slightly increased in EGFP expressing cells (Fig 2D).
These differences might reflect variations in the basal levels of endogenous PA28γ.
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