A basal level of Bcl-2 expression was detected in spinal cord from sham-operated mice (Figures 10a, 10a1 and 10b, 10b1).
A basal level of Bcl-2 expression was detected in samples from CIR rats.
A low basal level of Bcl-2 expression was detected in gingivomucosal homogenates from tissue of sham-operated rats.
In the cell line NCI-H82, which expresses a low basal level of Bcl-2, most of the combinations were slightly antagonistic.
Furthermore, we studied the effect of Bcl-2 gene transfection on the chemosensitivity of a breast cancer cell line that normally expresses a low basal level of Bcl-2.
However, sPD cybrids exhibited increased basal levels of Bcl-2 mainly associated with increased targeting to mitochondria.
A similar finding was reported for another LTED breast cancer cell line, MCF-7 2A, MCF-7 2Apressed elevated basal levels of Bcl-2 and whichnitially rexpressedto elevateded apoptosis [ 34].
Consistent with data from other study involving sPD cybrids (30), we have found that our sPD cybrids exhibited significantly increased basal levels of Bcl-2 relative to CT cybrids (Fig. 7C and D).
In addition, by inhibiting the function of BCL-2 protein by Navitoclax we showed that increased basal levels of BCL-2 are responsible for the resistance of GLI2ΔN keratinocytes to UVB-mediated apoptosis.
By contrast, a strong correlation was observed between basal levels of Bcl-2 and EBV status: all latency III EBV cells contained high levels of Bcl-2, whereas EBV cells had low levels of this protein.
