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Interestingly, without the palmitate treatment, Yap1 knockdown did not influence the basal expression level of CTGF (Fig. 4E and 4F).
They displayed a basal expression level of endogenous defense genes and stronger and quicker defense responses during fungal infection (Ozgonen et al. 2009).
Basal expression level of proteins related with DNA repair and cell-matrix adhesion were characterised by Western blots.
The low RpoS level detected in Kim53ΔnlpDL (Fig. 2) probably represents the basal expression level of the nlpD-rpoS bicistronic message transcribed from Txn3 (see Fig. 3C).
The basal expression level of α-SMA, FSP1 and FAP was significantly lower (P<0.01) in CAF-43 cells than in normal FB-43 fibroblasts (Figure 2A).
A complete denervation of the autonomic input to the liver had no overall effects on the basal expression level of clock genes and output genes.
We have observed a similar high basal expression level of Cyclin T1 in the CD4+ T cell lines CEM and H9, and this basal level is not further induced by activation (unpublished results).
We reasoned that a higher basal expression level of cation channels would render cells more susceptible to the channel blocking effect of amiodarone HCL seen in the GSEA data.
Despite variability in the basal expression level of CXCL12, 6 of 8 primary stromal cultures also showed significantly decreased expression of CXCL12 at 48 hours after transfection (Figure 3D).
A plot of CV of the basal expression level of rel as a function of time (Figure 3C) showed a similar variation as the transition rate, with the maxima in both the curves occurring around the same time.
These observed differences in the basal expression level of IGF2 suggest that the populations of hMSCs may have a different methylation status at the H19/IGF2 ICR, possibly resulting in mono-allelic expression in some cases and bi-allelic expression in others.
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