We measured basal oxygen consumption rate, and oxygen consumption rate after ADP addition, and after oligomycin addition.
After 1 day in galactose culture, basal oxygen consumption increased 16% (P = 0.24), and by 5 days, basal oxygen consumption increased 40% (P = 0.042; Figure 1D).
Thus, the increase in basal oxygen consumption after galactose culture appears to be driven primarily by increased mitochondrial oxygen consumption and electron transport flux rather than increased mitochondrial content, although specific mitochondrial proteins, such as SDH, are increased.
Other studies [ 16] also suggested that there was depressed basal O2 consumption by myocytes suspended in medium, decreased calcium uptake by mitochondria, and reduced mitochondrial membrane potential from diabetic rats.
No significant tetracycline treatment effect on basal oxygen consumption was found following induction of 2008 cells, confirming that tetracycline did not affect oxygen consumption in our model (data not shown).
The mitochondrial basal oxygen consumption rate (OCR-basal) was significantly lower in RCC4-EV cells than in RCC4-VHL cells under 20% O2 conditions (Fig. 4a).
We next measured key bioenergetic parameters of the OXPHOS pathway and found that the basal oxygen consumption rate (OCR) of NaBt-treated PC12-ND6 cells increased by 78% when compared to that of control PC12-ND6 cells (Fig. 2d, e).
Pooled data from all experiments (Fig. 3c,d; Supplementary Fig. 5c,d) revealed a 25% decrease in basal oxygen consumption rate (OCR) and a 42% reduction in maximum uncoupled respiration in the Leigh syndrome patient cells compared with the mean of the control cell lines.
To determine the regional cell density distribution and basal oxygen consumption rates (based on tissue volume and cell number) of temporomandibular joint (TMJ) discs and further examine the impact of oxygen tension on these rates.
