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These experiments were repeated using gentamicin (200 mg/kg i.p ., and no differential gentamicin immunofluorescence was observed between sound-exposed and control ears, nor between middle and basal coils (data not shown).
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The mechanism by which coiled coils may promote antigenicity is unclear, but Howard et al. [ 29] showed that a region at the amino terminus of the autoantigen histidyl-tRNA synthetase (which Coils [ 30] predicts to be a strong coiled coil (data not shown)) may promote autoimmunity by activation of dendritic cells.
Additionally, the NEDD1 CTD is predicted to have a coiled-coil structure (data not shown), therefore we next assessed whether this protein is multimeric in cells.
Importantly, Bb and Bb-CM alone did not modify the cytokine profile observed under basal conditions (data not shown).
However, both ovarian carcinoma cell lines express similar EGFR basal level (data not shown).
On the other hand, there was no difference under the basal diet (data not shown).
After anaphase and cytokinesis, both daughter nuclei move toward the basal side (data not shown).
LatrunculinA and jasplakinolide had no effect on basal apoptosis (data not shown).
In PTC, the staining was sometimes concentrated in both apical and basal region (data not shown).
All these treatments had no effects on basal VEGFR2 phosphorylation (data not shown).
We also tested the Alanine zipper motifs from other organisms (Figure 5A), but none of them was predicted to form Leucine zipper-like coils either (data not shown).
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