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All the basal category or control mice were injected (i.p) with 100 µl of sterile saline (PBS).
Many of the conventional luminal patients were reclassified as Basal by BluePrint, enlarging the Basal category while the pCR rate was maintained.
The low p53 expression in this cluster is atypical in the basal category and the very high expression of luminal cytokeratins justifies its grouping with the luminal class.
The basal category, with a frequency of 9.95%, represented a pattern harmonious with Bhargava et al. (10.7%) [ 20], Cheang et al. (9%) [ 26], and Tamimi et al. (10.9%) [ 18].
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BluePrint molecular subtyping reclassifies 22%% (94/426) of tumors, reassigning more responsive patients to the HER2 and Basal categories while reassigning less responsive patients to the Luminal category.
This reclassification of patients leads to an improved distribution of response rates in the different subgroups of patients: a lower pCR rate for BPLuminal patients compared with IHC/FISH-defined conventional luminal patients, with more responsive patients reassigned to the HER2 and Basal categories.
Filtering on a P-value less than or equal to 0.05 returned 14 basal cell categories and 23 luminal categories (Supplementary Table S3).
However, the basal-like category is composed almost entirely of triple-negative breast cancers (TNBCs) (i.e., tumors lacking ER, progesterone receptor (PgR) and HER2 expression) [ 3- 5].
Cluster analyses of microarray expression profiling data have shown that familial BRCA1 mutant tumors tend to fall into the basal-like category [ 2].
The majority of cases (61%) were of the luminal subtype (54.3% luminal A, 6.5% luminal B), whereas the basal-like category contributed 22%, 11% were in the HER2 subgroup and 7% were unclassified.
The four-category classification collapsed the basal and limited categories into a sedentary (< 5,000 steps/day) category, while the active and highly active categories were collapsed into a single active (≥ 10,000) category.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com