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With the sequencing of three related Caenorhabditis species it will be interesting to learn of the fates of bas-1 and the bas-1-like genes in other lines.
In order to assess whether the bas-1-like genes C05D2.3 and F12A10.3 might be under reduced selection pressure, we calculated the ratio of non-synonymous to synonymous substitutions (KA/KS) comparing the bas-1 orthologs and bas-1-like genes.
Therefore, tm351 is a fifth mutant allele of the bas-1 gene, and C05D2.4 corresponds to the gene bas-1.
The proportion of observed to potential non-synonymous substitutions (pN) among the bas-1-like gene comparisons was similarly much higher than for the bas-1 orthologs.
We found that the bas-1 genes are under purifying selection (KA/KS = 0.039), but the bas-1-like genes appear to be under reduced selective pressure; the average KA/KS for comparisons with bas-1-like genes was 0.148, more than three times the value of the bas-1 ortholog comparison.
Our analysis of synonymous vs. non-synonymous substitutions indicates that the bas-1-like genes C05D2.3 and F12A10.3 are under relaxed selection relative to bas-1 and other AADCs.
The tiny Republic of Kiribati (pronounced "KIR-i-bas"), 1,300 miles southeast of Honolulu, decided to change its time zone so it could be the first to claim the New Year.
The bas-1 gene is one of six AADC-like sequences in the C. elegans genome, including a duplicate that is immediately downstream of the bas-1 gene.
The adjacent AADC-homologous gene, C05D2.3, is not needed to rescue bas-1 mutants.
*Includes all pairwise comparisons (n = 5) except C.e. vs. C.b. bas-1.
Two DA synthesis genes, bas-1 and cat-2, are expressed in the male socket cells.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com