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Assessment of the barrier function of Caco-2 epithelium was assessed by TEER measurement and showed a major loss of barrier integrity after exposure to Fipronil and its metabolites, but not to Ethiprol.
We then assessed NAD(P H oxidase (NOX) activity and expression and the barrier function of Caco-2 cells.
In addition, mucus has a protective function for bio-relevant fluids present in the lumen of the gut, and direct exposure of Caco-2 cells (without a mucus layer) to lumen content stimulants reduces the barrier function of Caco-2 cells models (Ingels et al. 2002).
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However, recent study has also revealed that V. parahaemolyticus could profoundly disturb epithelial barrier function in Caco-2 cells with other virulence factors, in the absence of TDH [ 8].
Since we did not detect a reduced MTP activity in total lysates of Caco-2 cells in which Arfrp1 expression was suppressed, we conclude that ARFRP1 does not modify MTP function.
Figure 8 Cell viability of Caco-2.
Figure 2 Representative proteins silver stained gel images of Caco-2 cells.
Alkaline phosphatase activity was determined in the cytosol of Caco-2 cells.
Fig. 3 TEER values of Caco-2 cell monolayers at different time points.
The results showed satisfactory adhesion ability of free cells to a monolayer of Caco-2 cells (> 1000 CFU/100 Caco-2 cells for wet cells).
Figure 6 Cell viability of Caco-2 cells treated with different concentrations of nanoliposomes and Lf.
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