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Finally, in response to LPS, FAK silencing markedly impaired neonatal PEC barrier function, while FAK over-expression preserved adult PEC barrier function.
Stomach barrier function was assessed using a FITC-labelled dextran method.
In order to disrupt barrier function, LPS was added at a concentration of 1 µg/ml20.
Our data suggest that HDAC1 and HDAC2 regulate differentiation, barrier function and stress responses in enteroids.
Pre-treatment also significantly enhanced barrier function and decreased TNBS-induced inflammation (Fig. 5e, f).
Forster, C. Tight junctions and the modulation of barrier function in disease.
Qiu, Y. & Yang, H. Effects of intraepithelial lymphocyte-derived cytokines on intestinal mucosal barrier function.
Madara, J. L. & Stafford, J. Interferon-gamma directly affects barrier function of cultured intestinal epithelial monolayers.
To detect differences in barrier function (measured as change in resistance), Δ% values were calculated.
However, conditioned medium from LPS-stimulated A549 cells regulated barrier function in a different manner.
Shen, L. et al. Myosin light chain phosphorylation regulates barrier function by remodeling tight junction structure.
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