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A yellow barrier filter was used to enhance the contrast.
A barrier filter was placed between each camera sensor and lens to detect GFP expression.
No barrier filter was used to direct the reflected light by the sample to the detector.
The signal-to-noise ratio of the Canon camera was 16.1 when the yellow barrier filter was used (Fig. 9A), and 13.3 when both the cyan and yellow filters were combined (Fig. 9B).
The filter cube containing a single yellow dichroic barrier filter was effective in blocking reflected blue light, and allowed detection of both red chlorophyll fluorescence and green GFP fluorescence (Fig. 2H).
When only a single yellow dichroic barrier filter was used with the filter cube attached to the CCD-containing Nikon D80, EGFP fluorescence was only detected in the green channel (Fig. 7C).
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A barrier filter is used to detect autofluorescence and reflected light with wavelengths of 500 630 nm only.
Blue light, using a I3 filter block (450 490 nm excitation filter and 515 barrier filter), and green light, using a N2.1 filter block (515 560 nm excitation filter and 590 barrier filter), were used to detect the distribution and concentration of autofluorescent cyanobacterial or algal pigments.
A Kowa Genesis small animal fundus camera equipped with appropriate excitation and barrier filters was used to obtain fluorescein angiograms at early (90 s after fluorescein injection) and late (7 min) phases of dye transit.
The inlet barrier filters are part of a large category of filtration systems.
As such, and because the green barrier filters is a long pass filter (LP 516), the Alexa 594 fluorescence emission is still detected.
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