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Detection was with a band-pass emission barrier filter.
To investigate the benefit of the blue-free barrier filter (BF filter) in diagnosing dry eye.
Filter sets used for AO fluorescence observations are as follows: a BP450-490 exciter filter, and FT510 chromatic beam splitter, and an LP520 barrier filter.
The filter used for the acquisition was GFP2 consisting of a 460 40 nm band-pass excitation filter and a 510 nm barrier filter.
Finally, acridine orange (AO) was added onto specimens and observed using Nikon Eclipse E600 Epifluorescence microscope (excitation filter BP 490; barrier filter O515).
The emitted light above 500 nm was detected with a barrier filter.
We next carried out an in vitro invasion assay using a Matrigel coated barrier filter.
Separation of excitation and fluorescent light was done with a barrier filter at 500 nm.
Angiography was performed using s.c.-injected fluorescein dye and a blue (488 nm) laser stimulus with a barrier filter at 500 nm, and indocyanine green using an infrared laser stimulus (795 nm, barrier filter at 800 nm).
The sample was viewed under a fluorescence microscope using a 495 nm excitation filter and a 515 nm barrier filter.
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