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This was done by first reconstructing the barrel pattern for each animal from cytochrome oxidase stained layer 4 sections.
Slices from cortical layer 4 (400–900 µm) were processed with the Cytochrome oxidase staining to reveal the barrel pattern.
Outlines of the somatosensory isomorph were drawn by overlaying the barrel pattern revealed in the sections from each rat.
The barrel field was either visualized by cytochrome oxidase histochemistry, or the barrel pattern of the micrograph of the native slice was transferred manually into the reconstruction.
Two-dimensional outlines of the layer 4 barrel pattern were reconstructed for each animal from cytochrome oxidase stained layer 4 sections.
The location of the center of the injection site was marked in layer 2/3, and the aligned stack then used to plot its approximate position on the reconstructed layer 4 barrel pattern.
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Average barrel patterns were then calculated from the individual reconstructions for both control and paired groups.
3D contours and barrel patterns (see below) were visualized using Amira software (Mercury Computer Systems, MA).
The locations of all control and DE-paired injection site centers relative to average barrel patterns for both groups are shown in Figure 6 A. As the 2 groups appeared to have differing spatial distributions in the C-to-E axis, we designed a method to quantitatively compare the 2 distributions.
The anisotropy decay curve of VCV and VAV were virtually identical consistent with Amber having the same β-barrel folding pattern of Cerulean [14].
"We are not quite staring down the barrel, but the pattern is clear, the recovery out of the recession for the advanced economies will be difficult," Mr Cameron said.
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