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Each barrel comprises three tiers: upper, middle and lower tier.
PagP is an eight-stranded 20.2 kDa β-barrel OMP whose role in vivo is to transfer a palmitate chain from a phospholipid to lipid A. The larger OmpT β-barrel comprises ten β-strands (33.5 kDa) and functions as an endopeptidase.
The long strand β6 contains a kink that enables it to hydrogen bond to β5 to form a second half of the β-barrel comprising β6-β5-β1-β2 β6-β5-β1-β2 β6-β5-β1-β2 β6-β5-β1-β2onds to both β2 and β4 thecomplete the barrel (Figure 1).
Structurally, all SH3 domains share a common β-barrel fold comprising five β-stands, which are organized into two β-sheets.
Structurally, all SH3 domains share a common β-barrel fold comprising five β-stands.
One sub-group of PTases specific to secondary metabolism are the ABBA PTases, named in reference to the ααββ structural repeats that form a large β-barrel fold comprising the active center of the enzyme.
Determination of the crystal structures of wild-type GFP and Thr65-GFP revealed a β-barrel structure comprising 11 β-strands with lids flanking both sides and an internally positioned α-helix that harbors the chromophore (Yang et al. 1996; Ormö et al. 1996).
The Tud1 domain forms a canonical Tudor barrel that comprises five highly twisted antiparallel β-strands with a single 310 helix residing between strands β4 and β5.
TIM barrels now comprise the catalytic domains of more than half of the assigned GH families, and catalyse a much larger variety of GH reactions than any other catalytic domain architecture.
Structure determination by x-ray crystallography revealed the protein Cass2 to be a monomer organised into a barrel-like form comprising an antiparallel β-sheet of eight strands (Figure 4a).
In solution, apo-BCCP (E. coli, Pyrococcus horikoshii) is a flattened β- barrel structure comprising of two four-stranded β sheets [12], [13].
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