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Information about the organization within the barrel column is lacking.
Silicon probes were inserted into the C2 barrel column (O'Connor et al., 2013).
Neurons recorded in the C2 barrel column showed brief responses to touch.
Here, these tools were applied to reconstruct a 3D model of a cortical barrel column.
Specifically, it addresses the question how the different modules of the barrel column shape the response to whisker stimulation.
In consequence, our estimated numbers of L6cc and L6ct pyramids per barrel column are likely an overestimate.
To address this, electrode penetrations were made in barrel columns immediately adjacent to the spared barrel column.
The 0.5-mm slice thickness approximates to the diameter of one barrel column in SI (Riddle and Purves 1995).
A barrel column primarily processes sensory input from a related principal whisker (PW) on the animal's snout (Welker 1976).
Reconstructing the 3D structure of thalamocortical circuits between VPM and excitatory neurons in a barrel column yielded 5 major insights.
Neuron tracings were performed on 50- or 100-μm thick vibratome sections, cut approximately tangential to the D2 barrel column.
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