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Those two protein bands their exciting was very clear in treated barley leaf tissue.
Nuclei were prepared from barley leaf tissue as described above for Hi-C, before biotinylating the isolated chromatin using EZlink Iodoacetyl-PEG2-Biotin.
We have observed low levels of powdery mildew, spot blotch, scald and trace levels of barley leaf rust in New York.
In total, 11 BINs harbored a Prx based marker and a peak marker for basal resistance to barley leaf rust.
For example, Prx Profiling markers in BIN 2H_15.1 are associated with QTLs for resistance against barley leaf rust, barley mildew and two heterologous rusts (Puccinia persistens and P. triticina).
QTL positions in five of the populations composing the integrated map (Table S4) were used to test for association between Prx based markers and resistances to barley leaf rust [13], [14], [32], [33], to barley powdery mildew [22], [34], and to heterologous rusts ([14], [35] and unpublished QTLs by Jafary and Niks and Alemu and Niks).
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They include: cereal rye, winter barley, velvet leaf, ragweed, radish, white mustard, crimson clover, red clover, triticale, pearl millet, giant foxtail, hairy vetch, and annual ryegrass.
This enabled reconstruction of the single barley organs leaf and stem as well as the architecture of the whole barley plant on the decimetre scale.
For Barke and the wild barley accessions leaf material from three to four plants was used to create bulked samples.
The ability of N- HvGR-RBP1 and FL- HvGR-RBP1 to bind to an ssRNA affinity chromatography column composed of RNA isolated from barley plant leaf samples was also tested.
Barley resistance to leaf rust pathogens is governed by major resistance (R) genes (Rph genes) that are race-specific.
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