When using transformed yeast expressing a trichothecene 3- O-acetyltransferase in small-scale barley fermentations, DON contaminating the ground grain was converted to 3ADON, and thereby the concentration of DON was reduced in DDGS.
Data were analyzed from 96 small-scale barley-fermentation mashes prepared from five barley genotypes VA06H-255, VA04B-125, Thoroughbred, Price and Eve).
Mr. Clark's new set of challenges involves hops, barley and fermentation.
The current study aimed to increase the digestibility of dried distillers grains with solubles (DDGS), based on 80% wheat and 20% barley, by fermentation and enzyme addition.
To our knowledge, this is the first detailed report of yeast expressing a DON modification enzyme during barley ethanol fermentation, and provides a basis for evaluating novel detoxification enzymes such as DON de-epoxide hydrolases to reduce DON in DDGS in the future.
To our knowledge, this is the first detailed report of yeast expressing a DON modification enzyme during barley ethanol fermentation, and provides a basis for evaluating novel detoxification enzymes such as DON de-epoxide hydrolases to reduce DON in DDGS [ 22, 27].
There may be several reasons for the different levels of conversion in our barley ethanol fermentations compared with the previously published feeding assays.
In this study, we tested the hypothesis that two of these enzymes (FgTRI101 and FfTRI201) would reduce DON in DDGS resulting from a series of small-scale barley ethanol fermentations.
Six ruminally fistulated steers were used in a completely randomized design to determine the effects of feeding mint silage or barley silage on ruminal fermentation and nutrient utilization.
Eight crops that often be investigated as substrates for solid fermentation, namely barley, buckwheat, brown rice, polished rice, red beans, soybean and green beans (mung bean) were examined in this study (Yang et al. 2013).
