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The impregnated bark was dried at 160 °C till complete charring for another 4 h.
The bark was dried at room temperature and then stored at −20°C.
The stem bark was dried at room temperature and pulverized.
The bark was dried at 40°C in an incubator with forced air circulation apparatus for 48 hours.
The aqueous extracts of stem bark were prepared as follows: Direct aqueous extract (DAE): The stem bark was dried at 55°C overnight.
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The stem bark were dried at room temperature and powdered in a knife mill (0.86 µm), resulting in a mass of 651.52 g of plant material, which was submitted to maceration at room temperature with ethanol 70% for a week with daily shake.
The D. alata barks were dried at 37°C over 48 hours and then powdered, ground in a mill, macerated (200 g, during 5 days) in 2 L of 70% ethanol and the suspension was then percolated (under protection against light) at 20 drops/min, resulting in a 20% (m/v) hydroalcoholic extract [ 10].
The soluble-free bark was dried in a convection oven at 60°C for 3 days, milled on a Wiley mill and passed through a 40 60 mesh sieve.
The washed A. indica bark was dried in sun for 15 days.
The stem bark of S. pinnata was dried at room temperature for 7 days, finely powdered and used for extraction.
The stem bark of A. adianthifolia was dried at room temperature (25 ± 2°C) for three weeks and crushed.
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