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In contrast, the non-neoplastic MCF10A cell line barely expresses ANT2 as well as ANT1 but highly transcribes ANT3.
Since the cell line barely expresses endogenous 17β-HSD1 [ 11, 15], we used it as cell model for the increase of 17β-HSD1 expression.
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Curiously, in the non-tumourigenic PZHPV-7, amplification of EEF1A1 and EEF1A2 genes was detected, even though these cells that barely express EEF1A2 and EEF1A1 expression did not result to be higher than in cancer cells.
In limb buds, the transgene is barely expressed at NF stage 51, and expression remains weak until NF stages 61 62.
Although barely expressed in most normal tissues, deregulated expression of Id proteins has been demonstrated in a variety of human tumours (Lin et al, 2000; Schindl et al, 2001; Wang et al, 2004).
Neuronal cadherin and α9-integrin were selected for their downregulation under GSI treatment, their significant expression (other proteins downregulated but barely expressed in control samples were ruled out) and their previously well-established involvement in cell adhesion, motility and metastasis.
The C. forskohlii GGPP synthase gene was strongly expressed in the leaves, whereas expression was decreased in stems and barely expressed in roots (Fig. 5).
While barely expressed under basal conditions, immunofluorescence demonstrated widespread expression in the nucleus of TH expressing chromaffin cells in the adrenal medulla after IMO stress [ 26].
In this regard, it is noteworthy that Mamld1 is clearly expressed in fetal Leydig and Sertoli cells and is barely expressed in adrenal cells [1], [13], and that Cyp17a1 expression is indispensable for T production in Leydig cells [14].
After selecting HM3KO as a highly expressing c-RET cell line and G361 as a barely expressing c-RET cell line, we confirmed GFRa1 protein expression in the cells.
The DFR2 gene was over-expressed in flower petals of T369 but it was barely expressed in T321.
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