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In germinating seeds, the transcripts of BdDof2, BdDof4, BdDof8, BdDof16 and BdDof19 are barely detected (data not shown).
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In addition, CaMKKβ was not detected (data not shown).
Interestingly, IL-17 production was not detected (data not shown).
The expression of Bcl-xs was barely detectable (data not shown).
Alternatively, while CAV1 expression was readily appreciable in most endometrial tissues, caveolin-2 was barely detectable (data not shown).
Moreover, after 6 and 9 hours from LPS exposure, α-SMA+ ECs were barely detectable (data not shown).
In SKBr3 cells, which carry an amplification of the ERBB2 locus, the endogenous MBP-1 protein was barely detectable (data not shown).
From the genomic data, we inferred that Gypsy and DIRS1 LTR retroelements had expanded recently in the copperhead, and transcriptional data show moderately high levels of both of these in the copperhead (at 0.54% and 0.18% of transcript reads, respectively) yet these were either barely detected or not detected at all in the python transcriptome (fig. 7).
As p-SMAD2C was barely detected or not observed in DU145 cells after incubation with control medium or chelators, TGF- β (10 ng ml−1) was used as a positive control and demonstrated that p-SMAD2C was significantly (P<0.01) induced.
Interestingly, in the cytoplasmic region of those infected cells hnRNP-K was barely detected and colocalization percentages were not significant (data not shown).
We also probed RNA from mouse serum with a probe complementary to the 5′ end of tRNA-Asn-GTT to confirm the low abundance of circulating tRNA halves derived from tRNAs that were barely detected in the sequencing data.
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