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A COI based barcoding effort based on specimen from North Western Europe resulted in five "Molecularly defined Taxonomic Units" (MOTU1-5), sofe of which could be attributed to existing species [ 23].
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Certain gene regions of the five barcoding markers based on GenBank annotations were then obtained.
In addition, nuclear genes can provide more information than barcoding based on organellar DNA, which is inherited from only one parent [30].
However, a DNA barcoding approach based on cox1 sequence diversity has been utilized for identification of closely related species [ 51].
A DNA barcoding approach based on cox1 sequence diversity has been utilized for identification of closely allied species [ 43].
We describe a novel method of species identification, fluorescent fragment length barcoding, based on length variation in regions of the 18S and 28Sα ribosomal DNA.
Table 2 shows the comprehensive barcoding identification results based on GenBank and BOLD databases.
DNA barcoding based on cox1 has been successful in species identification across a wide array of taxa [9] [12].
The most successful molecular approach in this field is DNA barcoding based on cytochrome c oxidase I (COI) marker, but other markers are used as well.
Barcoding strategies developed based on direct labeling of cells using maleimido-monoamide-DOTA (m-DOTA) provide a very useful tool.
Herein, we put forward an innovative barcode system based on the specular reflection of NAA.
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