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The reverse primer was barcoded with a unique 6 bp error-correcting to each sample.
Each library is barcoded with a unique 6-mer index located on the 3′ linker.
Samples are distinguished by their locations in the multidimensional sample pool, each pool barcoded with a unique short DNA sequence.
To summarize, DNA from each individual was digested with SbfI and then barcoded with a unique 6-base sequence 3′ to the SbfI cut site.
To this end, each cDNA library was barcoded with a unique 10-base sequence (MID, Multiplex IDentifier) that is recognized by the sequencing analysis software, allowing for automated sorting of MID-containing reads.
As each tissue is barcoded with a unique number, which allows tracking of the animal from which the tissue originated and the animal house, it is possible to identify the source of any problem arising with the quality of the tissues and promptly rectify it.
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Each pool is barcoded with a short unique sequence before being fed into a SOLiD 5500 sequencer.
The two forward primers were not multiplexed in a single reaction, but amplified separately for each of the 16 samples, necessitating 32 total reaction cocktails, each with a unique barcoded forward primer.
M2 seed was planted in a row by column grid format, and M2 plants were individually tagged with an assigned barcode as a unique identifier.
Serum and plasma specimens will be split into 0.25 ml aliquots, labelled with a unique barcode.
Each shRNA was tagged with a unique barcode, enabling subsequent identification by high-throughput sequencing (HTS).
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