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Each pool is barcoded with a short unique sequence before being fed into a SOLiD 5500 sequencer.
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Samples are distinguished by their locations in the multidimensional sample pool, each pool barcoded with a unique short DNA sequence.
The reverse primer was barcoded with a unique 6 bp error-correcting to each sample.
When these primers were not successful, the primer cocktail C-tRWF_t1 (see Table 1) enabled amplification of the standard 658-bp barcode region together with a short upstream sequence in an additional 15% of the specimens.
DNA barcoding utilizes a short, standardized gene region to identify species; this study uses the 658 bp region of the cytochrome c oxidase I gene (COI), chosen as a DNA barcode for animal life [ 7].
Jones with a short putt.
Start with a short slackline.
Start with a short pencil.
A DNA "barcode" refers to a short region of a gene that changes over evolutionary time at a rate that results in measurable distinctions among species (analogous to the barcodes on products in stores).
Given the above results with a pilot barcode microarray, we constructed a short unmodified barcode probe array with 13K unique features (which hereafter is referred to as the "SUBarray").
Another $1 million will support DNA barcoding, a short-cut way to identify species.
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