The reverse primer was barcoded with a unique 6 bp error-correcting to each sample.
The nCounter "code set" contains a pair of ∼50 nt biotinylated DNA probes that are barcoded with different combinations of fluorescent tags.
Next, barcoded Illumina sequencing libraries with a different barcode used for each sample were constructed from the fragmented DNA according to the protocol described in Meyer and Kircher [ 21].
Sets of 48 or 96 digested DNA samples, each with a different barcode adapter, were combined (5 µL each) and purified using a commercial kit (QIAquick PCR Purification Kit; Qiagen, Valencia, CA) according to the manufacturer's instructions.
For multiplex sequencing, DNA samples, each with a different barcode, are pooled, randomly sheared, size selected (300 700 bp), and a second adapter is ligated after polishing and filling ends.
Each library was tagged with a different barcode at the 5' end to enable multiplexing during sequencing.
In FCB, each sample is labeled with a different signature, or barcode, of fluorescence intensity and emission wavelengths, and mixed with other samples before antibody staining and analysis by flow cytometry.
Most barcodes on products sold in the U.S. and Canada are UPC barcodes, but be wary of compressed 6-digit UPC barcodes, which have a different, more complex encoding system.
Therefore, this Review focuses on aggregate prevention by HSPs and will reveal that each of these proteinopathies is associated with a different pattern or 'barcode' of rescue depending either on the HSR or individual HSPs.
Some exceptions are made for barcodes with a single reads and barcodes that are not of the expected length, allowing for more aggressive clustering of these barcodes with other barcodes.
