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Chen et al. first developed ITS2 as a DNA barcoder of medicinal plants, established a plant barcode identification system that was mainly based on ITS2, and used psbA-trnH as a complementary barcode.
Found in the hindgut of Coptotermes cf. acinaciformis with a distinct barcode identification (KJ438378).
In the present work we introduce a novel technique for tracking the dissemination of food within an ant colony by combining the imaging of fluorescently labeled food with the video tracking of ant trajectories using 2D barcode identification.
The balance of within- and between-species sequence variation is an important aspect of barcode identification [1] [2], [29] and should be taken into account in the development of a barcode for any group of organisms.
In addition, we detected no instances of sample switching due to aberrant barcode identification.
If a hypothesized morphospecies contained several lineages based on the COI clustering and barcode identification, further specimens were sampled.
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This result is similar to other barcode studies which have demonstrated that including more than two or three loci does not significantly increase the power of assignment in barcode identifications [22] [24].
Sphingidae is the target of a global barcoding campaign [ 15] and shows high success for species-level barcode identifications.
Sonet, G. et al. Adhoc: an R package to calculate ad hoc distance thresholds for DNA barcoding identification.
Table 2 shows the comprehensive barcoding identification results based on GenBank and BOLD databases.
Therefore, a rapid and simple DNA barcoding identification system, even an imperfect one, is likely to be welcomed.
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