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This plasmid retains a strong gpdA promoter to drive the insert's gene expression and Bar resistance coding sequence to express the selectable marker that would provide resistance to phosphinothricin (PPT).
These include 11 ORFs representing a wide spectrum of biological functions like immunoglobulins (IgG1 heavy and light chain), structural viral proteins from BTV (Blue tongue virus) and PVX (Potato virus X), the silencing inhibitor p19, the bar resistance marker and GFP.
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Plasmid JS101 containing the bar herbicide resistance selectable marker gene regulated by the rice actin 1 (Act1) promoter was used as a selectable co-transformation marker.
The T-DNA construct contains a CaMV-35S promoter-driven sGFP ORF with the NOS (nopaline synthase) terminator, and NOS promoter-driven bar (bialaphos resistance gene) [45] ORF with the Arabidopsis rbcS (rubisco small subunit) terminator.
bar confers resistance to phosphinothricin (Pall 1993).
Additional binary Cas9 vectors were produced by replacing nptII from p201NCas9, with hph, bar (phosphinothricin resistance), or GFP.
The properties of mortars/concrete made from geopolymeric binders are discussed with respect to fresh and hardened states, interfacial transition zone between aggregate and geopolymer, bond with steel reinforcing bars and resistance to elevated temperature.
Barring armed resistance, diplomatic options remained — the Lithuanian government could have rejected the ultimatum, retreated abroad, and formed a recognized government-in-exile.
According to design magazine Core77, the WREX is an "assistive device made of hinged metal bars and resistance bands that enables people with underdeveloped arms to play and feed themselves".
A ProMAS-BAR-35Ster resistance cassette flanked by EcoRI-sites was amplified from genomic DNA of a SAIL line containing a T-DNA derived from pDAP101 into the EcoRI site of the minimal pCAMBIA to generate pCAMBIA-proMAS-BAR-35St (pCMBAR).
Barring the massive resistance of an industry resisting its own demise, of course.
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