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Ten optimal spectral bands were selected using the algorithm, with six out the selected bands within the same near-infrared spectral region.
The relative expression levels were normalized using tubulin bands within the same linear range of detection.
The relative expression levels for the dystrophin bands were normalized using tubulin bands within the same linear range of detection.
Moreover, neighboring frequency bands within the same neuronal network may compete or interact with each other [ 20].
The heterogeneity of association in different age bands within the same study argues strongly that these effects are real.
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Some users may select the same band within the same time slot.
To correct for inconsistencies in protein loading, each PNPLA2 band was normalized to the β-tubulin band within the same lane.
The normalization was performed relative to a control band within the same lane whose intensity was considered to be independent of guanine.
This technique ensures that the mean and standard deviation of PAN image and MS bands are within the same range, thus reducing the chromatic difference between both images.
Differences in intensities between bands were obvious within the same species for each primer combination but did not show any direct relation with the size of the amplified fragment.
Sample band densities were ratio-normalized by using β-actin band intensities, obtained within the same PCR reaction, with the aid of NIH Image J software (NIH, Bethesda, Maryland).
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