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DNA bands were visualized under UV light using a GeneRuler™ 1 kb DNA Ladder (Fermentas).
Electrophoresis was done in 1.5% agarose gels in 1 X TBE buffer, at 100V for 30 min. The gels were stained with ethidium bromide for 15 min. Bands were visualized under UV light.
cpDNA bands were visualized under UV illumination and then recovered.
RNA bands were visualized under an ultraviolet lamp.
Stained bands were visualized under UV light, and photographed with an Image Master VDS machine (Amersham).
After extraction with TRIzol (Invitrogen) following the manufacturer's instructions and agorose gel analysis, total RNA bands were visualized under UV light [30].
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The quality of the RNA samples was determined via electrophoresis through agarose gels and staining with ethidium bromide, the 18S and 28S RNA bands being visualized under ultraviolet light.
The DNA band was visualized under Gel documentation system (G BOX BioImaging System) (Syngene, USA) and the image was analyzed using a Genesnap software (Syngene, USA).
Due to the intercalating dye, Gelred (Biotium, Hayward, CA, USA), that was added to the gel pre-run, the DNA band was visualized under UV light and was then extracted.
DNA bands were visualized and photographed under UV light (340 nm).
The PCR products were analysed by electrophoresis in 2% agarose gels stained with ethidium bromide, and bands were visualized and photographed under ultraviolet excitation.
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