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Protein bands were visualized applying Coomassie Brilliant Blue R-250 and silver staining.

Bands were visualized applying chemiluminescence SuperSignal®detection system (PIERCE).

Anti-rabbit and anti-mouse IgG alkaline phosphatase-conjugated secondary antibodies (Sigma) were applied and the bands were visualized using SigmaFAST BCIP/NBT (Sigma).

Next, the horseradish peroxidase (HRP -conjugated goat anti-rabbit IgG secondary antibody (12-348; Millipore) was applied and immunoreactive bands were visualized by enHRP -conjugatedinescence usingoate Luminata Forte HRP Substrate (Millipore) and detected wIgG the G:Box secondaryyngene).

Reactive protein bands were visualized using LumiLightPLUS Western blotting substrate (Roche Applied Science, Penzberg, Germany) and a LAS-3000 lumimageent imanalyzeryzer (Fuji Photo Film (Europe), Kleve, Germany).

The bands were visualized using a Fuji 4000 imaging system (Fuji, Tokyo, Japan) after applying electrochemiluminescent (ECL) detection reagents (Santa Cruz Biotechnology).

Enhanced chemiluminescence (ECL) reagent (Millipore, Bedford, MA) was applied to the membranes and specific protein bands were visualized by FluorChem FC2 Imaging System (Alpha Innotech, San Leandro, CA).

Bands were visualized by enhanced chemiluminescence (Pierce Biotechnology).

Protein bands were visualized by ECL.

The bands were visualized by using Pierce ECL Western blot substrate (Amersham).

The bands were visualized by silver staining technique (Merril 1987).