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Protein bands were visualized according to standard ECL protocols.
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Bands on blots were visualized according to Li-Cor Odyssey standard protocols.
The immunoreactive bands were visualized by chemiluminescence according to the manufacturer's recommendations (Thermo Fisher, Waltham, MA, USA).
The cDNA bands were visualized by silver staining according to Bassam et al. [ 23].
After being washed with TBST, the blots were incubated with an HRP-conjugated anti-IgG and the target bands were visualized with ECL plus reagents according to the manufacturer's instructions.
Immunoreactive bands were visualized with ECL Plus (Amersham), according to the manufacturer's instructions.
Subsequently, the membrane was incubated with appropriate secondary antibody, and the immunoreactive bands were visualized using enhanced chemiluminescence kit according to the manufacturer's instructions.
Bands were visualized with an enhanced chemiluminescence kit according to the manufacturer's protocol.
The formed bands were visualized with the enhanced chemiluminescence method according to the manufacturer's instructions (GE Healthcare, Piscataway, NJ).
Following multiple washes in TBST, bands were visualized using Visualizer Western Blotting Detection Kit (Millipore, Billerica, MA) according to the manufacturer's protocol.
Membranes were washed two times in PBS-Tween, and specific bands were visualized using the ECL system (GE Healthcare) according to the manufacturer's instructions.
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