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Protein bands were visualised with Coomassie Blue stain.

Protein bands were visualised with the GelDoc scanner (Bio-Rad Laboratheies), using the fluorescent method of the WesternDot Kit (Life Technologies) and the primary antibodies bGFR (cat. no. F4305-08, USwampscottl, SwaMAscott, MA, USA), KDR (cat. no. SAB4300356, Sigma) and GAPDH (cat. no. NB300-327, Novus Biologicals, Cambridge, UK) as loading control (dilutions recommended by the producers).

The immunoreactive bands were visualised with chemiluminescence.

Protein bands were visualised with super signal reagents.

After washing, immunoreactive bands were visualised with the SuperSignal chemiluminescent substrate (PerkinElmer, Waltham, MA, USA).

Protein bands were visualised with SuperSignal® West Dura extended duration substrate (Perbio; http://www.perbio.com).

DNA bands were visualised by transillumination with UV and photographed using the Imagemaster with Fuji Thermal film (Pharmacia Biotech, Roosendaal, The Netherlands).

The resultant protein bands were visualised after incubation with HRP-labelled secondary antibodies, and reacted with substrates of a supersignal kit (#1856136, Thermo Scientific, Waltham, MA, USA).

Protein stability and quantity were check by 1-D Nu-PAGE (Invitrogen) and bands were visualised by staining with Coomassie Safe Blue (Pierce).

Bands were visualised by staining with ethidium bromide and digital images were captured following UV exposure on a Biorad Gel Doc 1000 system.

Protein bands were visualised by staining with Coomassie Brilliant Blue G (Sigma-Aldrich, UK).