Suggestions(2)
Exact(5)
After further washing in TBS-Tween-20, the membranes were incubated with an ECL plus reagent (RPN2132, Amersham) and protein bands were visualised on X-ray films.
Protein bands were visualised on Amersham Hyper-Max film by the ECL chemiluminescence system as recommended by the manufacturer (Amersham, Buckinghamshire, UK).
Bands were visualised on a 3% agarose gel containing SYBR® Safe DNA stain (Invitrogen).
Bands were visualised on a UV transilluminator, excised, placed in 15 μL of ddH2O and stored at -20°C for sequencing reactions.
Immunoreactive bands were visualised on Hyperfilm ECL (GE Healthcare, Chalfont St. Giles, Bucks, UK), and the predicted protein was confirmed by recombinant CA IX and HIF-1α that was run alongside the samples.
Similar(55)
Protein bands were visualised with SuperSignal West Pico Chemiluminescent Substrate (Pierce) on X-ray film (Agfa, Morstel, Belgium).
Bands were visualised either using X-ray film or on a Molecular Dynamics phosphor Imager [28].
This was washed a further three times, and bands were visualised using ECL chemiluminescent reagents (GE Healthcare) and developed on Biomax film (Kodak).
Protein bands were visualised with SuperSignal West Pico Chemiluminescent Substrate (Pierce, Rockford, IL, USA) on X-ray film (Agfa, Mortsel, Belgium).
Bands were visualised by staining with ethidium bromide and digital images were captured following UV exposure on a Biorad Gel Doc 1000 system.
Protein bands were visualised using enhanced chemiluminescence (GE Healthcare).
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