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Bands were visualised as described above.
Immunoreactive bands were visualised as reported elsewhere (Kawakami et al, 2002).
Immunoreactive bands were visualised as reported earlier (Aird et al, 2008).
These blots were subsequently re-probed with an antibody against total ERK and p38 (New England Biolabs, Inc .. Bands were visualised as before.
Bands were visualised as clear zones following staining with Coomassie blue (Leber and Balkwill, 1997).
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Protein bands were visualised and densitometry was performed.
Immunoreactive bands were visualised using Pierce ECL Western Blotting Substrate Fisher Scientificc, Loughborough, United Kingdom).
Protein bands were visualised using Immobilon Western Chemiluminescent HRP substrate (Millipore, Darmstadt, Germany).
Protein bands were visualised using Amersham Biosciences ECL Western blotting detection reagent (GE Healthcare) according to the manufacturer's instructions.
The immunoreactive bands were visualised using horseradish peroxidase-conjugated secondary antibody and enhanced chemiluminescence (Amersham).
Polypeptide bands were visualised using Simply Blue Safe Stain (Invitrogen, Paisley, UK).
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