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Bands were then resolved on an Odyssey scanner (LI-COR Biosciences).
DNA bands were then resolved in a 1.5 % agarose/0.5× Tris borate EDTA gel, electrophoresed at 50 V for 3 h, and visualized with FloroSafe stain (1st BASE).
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PCR fractions were then resolved on agarose gels, the ∼350bp amplification band excised, purified using a QIAquick gel extraction kit (QIAGEN), and cloned into the pGEM-T Easy Vector (Promega).
After the last cycle, samples were incubated for 5 min at 72°C. 15 μl of amplified products were then resolved on a 1% agarose gel, and DNA bands visualized under UV.
The amplified products were then resolved by gel electrophoresis.
The analytes were then resolved in 200 μL acetonitrile.
Equal amounts of protein were then resolved using SDS-PAGE.
The resulting radioactive complexes were then resolved by SDS-PAGE.
The released proteins were then resolved by SDS-PAGE.
The samples were then resolved using 10% SDS-PAGE.
Samples were then resolved on a 15% denaturing PAGE gel.
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