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The PAM fraction polypeptide pattern was very similar to that of the plasma membrane, but certain polypeptide bands were specific for one of the fractions only (Fig. 8, arrows).
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To further confirm whether these three bands are specific for the NF-κB complexes, a competition assay was performed.
This band is specific for NF-κB, as previously demonstrated [39], [32].
This band is specific for the polyclonal anti-human RANK Ab as shown in COS cells transiently transfected with a human RANK cDNA.
Immunoblotting of lysates from mammalian and human cell lines showed a major band which migrated at the predicted size of RASSF7 (34 kDa) and this band is specific for RASSF7 because it was reduced by two different knockdown approaches.
Some bands are specific constant markers for each taxon and can be discriminated bay their electrophoregrams.
Results confirmed that bands of PCR products were specific for the target genes and suitable for further examination.
In addition to the cullin proteins, no other bands were detected that were specific for the FLAG-CAND1 induced cells.
The polypeptide bands with the strongest intensity that were specific for the CS-A conjugated Sepharose were associated with C1q.
This band was detected with probes that were specific for the DNA from nts 1833 to 907 and not probes for the 3' end of DNA (Fig. 3D).
The band profiles of fish bacteria or fruit yeasts and fungi from different farms were specific for each location.
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