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Sixteen bands were resolved, with unresolved very high molecular weight vWF (> band 16) also visible.
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Seven major green bands were resolved upon solubilization of wild-type thylakoid membranes with 0.8% dodecyl-α-D-maltoside (α-DM) on Deriphat-PAGE [ 25].
Purified DNA was then digested with BstU I (New England Biolabs, Ipswich, MA, US) and the resulting bands were resolved on 1% agarose gel.
DNA bands were resolved on a 2% agarose gel containing ethidium bromide (0.5 mg/ml).
A total of 73 bands were resolved from the 11 A. ustus isolates.
T-RFLP bands were resolved over the region between 50 and 958 bases.
DNA bands were resolved and interpreted according to previously reported criteria [ 15].
Amplicons were resolved with 1% agarose gels, bands were excised, and DNA was purified by using a Wizard SV gel and PCR clean-up system (Promega, Southhampton, UK).
All disagreements were resolved with discussion.
Disagreements were resolved with JW.
Disagreements were resolved with consensus.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com