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DNA bands were resolved on a 2% agarose gel containing ethidium bromide (0.5 mg/ml).
Purified DNA was then digested with BstU I (New England Biolabs, Ipswich, MA, US) and the resulting bands were resolved on 1% agarose gel.
Protein bands were resolved on 10 20% Tricine SDS PAGE gels (Invitrogen, Carlsbad, CA), transferred to PDVF Immobilon-FL membranes (Millipore, Billerica, MA), and blocked for 1 h using a 5% milk solution prepared in phosphate buffered saline (Invitrogen, Carlsbad, CA) supplemented with 0.05% Tween 20 (PBS-T; Sigma, St . Louis MO), followed by an overnight incubation with primary antibody at 4°C.
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On average, 8.8 (SD 4.4) separate T-RF bands were resolved by Culture-derived Molecular Analysis per patient.
Sixteen bands were resolved, with unresolved very high molecular weight vWF (> band 16) also visible.
Seven major green bands were resolved upon solubilization of wild-type thylakoid membranes with 0.8% dodecyl-α-D-maltoside (α-DM) on Deriphat-PAGE [ 25].
A total of 73 bands were resolved from the 11 A. ustus isolates.
T-RFLP bands were resolved over the region between 50 and 958 bases.
DNA bands were resolved and interpreted according to previously reported criteria [ 15].
At least 11 chromosome-sized DNA bands (CB 1 through CB 11) for FSB, 6 bands for omFSB1, and 7 bands for omFSB2, respectively, were resolved on a CHEF gel.
PCR products were resolved on DNA gels and bands of expected size were extracted and purified using the GeneClean kit (MP Biomedicals, Santa Ana, CA) and ligated into appropriate plasmid vectors.
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