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The western blot bands were quantitated with ImageJ software (National Institutes of Health, Bronx, NY, USA).
The gels were dried, and the radioactive bands were quantitated with Storm 820 and Imagequant software (Amersham Pharmacia, Piscataway, NJ).
Radiolabeled bands were quantitated with a PhosphorImager densitometer (Molecular Dynamics) using IMAGE QUANT software to assess the ratio of probe bound to GliZF versus free probe.
Positive immunoreactive bands were quantitated with a densitometer LAS-30000, FUJIFILM, Tokyo, Japan) and normalized to the levels of total protein and β-tubulin.
The dried gels were visualised, and the radioactive bands were quantitated with a Universal Hood II (Bio-Rad Laboratories, Hercules, CA, USA).
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Labeled bands were detected by Luminata Crescendo Western HRP substrate (Millipore, Billerica, MA, USA) and images were captured and the intensity of the bands was quantitated with ImageReader LAS-4000 (Fujifilm CorporaTokyo, Japan Japan).
Labeled bands were detected by Immun-Star HRP Chemiluminescent Kit, and images were captured and the intensity of the bands was quantitated with the Bio-Rad VersaDoc image system (Bio-Rad, Regents Park, NSW, Australia).
Gels were vacuum dried, and Dvl1-3 protein bands were quantitated using a Typhoon phosphorimager with ImageQuant software (Molecular Dynamics).
Bands were quantitated using the integrated density function of ImageJ.
Bands were quantitated using National Institute of Health's ImageJ software.
Protein bands were quantitated by densitometry, and analysed using GeneTools software.
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