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EndoH treated and non-treated samples were then run on a 7.5% SDS-PAGE gel, transferred to nitrocellulose, and bands were quantitated following scanning on a Typhoon Trio Variable Mode Imager (GE Healthcare) and ImageQuant 5.1 to quantitate band intensity.
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For time course experiments, bands were quantitated in AIDA software and data fit in MicroCal Origin 7 software using the following equation: where a and k are constants representing the asymptote and the relative reaction rate, respectively.
Bands were quantitated using the integrated density function of ImageJ.
Bands were quantitated using National Institute of Health's ImageJ software.
Protein bands were quantitated by densitometry, and analysed using GeneTools software.
Gels were vacuum dried, and Dvl1-3 protein bands were quantitated using a Typhoon phosphorimager with ImageQuant software (Molecular Dynamics).
Immunoreactive bands were quantitated and expressed as the ratio of each band density to corresponding loading control (β actin) band density and values were represented after normalization to untreated control.
Bands were quantitated using reverse image scanning densitometry (Photoshop CS2, Adobe) by normalizing the band intensity of the co-immunoprecipitated protein to the band intensity of the immunoprecipitated protein.
Individual protein bands were quantitated by densitometry.
Radiolabeled bands were quantitated using a phosphorimager.
Bands were quantitated by laser densitometry.
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