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Protein bands were quantified from the membrane by densitometry using the Adobe Photoshop V7.01 imaging system.
The protein bands were quantified from these films by densitometry using Quantity One software (Bio-Rad, Hercules, CA).
Individual bands were quantified from the membrane images by densitometry using the Quantity One software program (Bio-Rad).
After three washes, protein bands were quantified from the membrane by densitometry using the Adobe Photoshop version 7.01 imaging system.
Mean relative intensities of the relevant protein bands were quantified from triplicates and normalized to beta-actin levels (Supplementary Figures S5 S7).
Bands were quantified from scans of film after short exposures, using Adobe Photoshop, and normalized against the intensity of β-tubulin or fatty acid synthase bands.
Similar(53)
Multiple serial dilutions of a reaction were fractionated alongside the undiluted reaction, and the substrate band was quantified from those that fell within the linear range of estimation.
The bands were quantified using ImageJ, version 1.43u (from National Institutes of Health, Bethesda, MD, USA; http://rsbweb.nih.gov/ij/).
Signal intensities from gel bands were quantified with the ImageGauge software (Fuji), and each sample was normalized to transcript levels of the A. aegypti ribosomal S7 gene.
Protein bands were quantified by densitometry using ImageJ 1.37v software from NIH (rsbweb.nih.gov/ij/).
Multiple exposures of each blot were used to obtain grey scale images and the GFAP (Pharmingen) immunoreactive bands were quantified with the Quantity One Analysis program from Bio-Rad.
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