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We found that even if some of the first round PCRs were not very successful (PCR product bands were not visible in ethidium bromide stained agarose gel), it usually did not affect the final full-length DNA amplification.
Samples were re-extracted if ribosomal bands were not visible.
These bands were not visible on a gel without substrate, excluding the possibility that they originate from kinase auto-phosphorylation.
Lysates from cells expressing either F-NACC1 WT or F-NACC1 (K483R and K498R) mutants displayed migrating bands, but these bands were not visible in lysates from K167R mutant (Fig. 3b).
1D-SDS-PAGE gel band patterns showed that anaerobic and microaerophilic cultures resulted in very similar protein expression profiles whereas in the aerobic culture sample some of the major bands were not visible.
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Suffix sequences should be present at the 3' ends of all poly(A)+ F element transcripts, but the corresponding bands are not visible against a background of much more highly abundant RNA molecules generated by the independent suffix copies (compare the left panel in Figure 2 with the right panel in Figure 3).
The other small AS5 bands are not visible in this gel system.
Indeed, as indicated in the schematic accompanying the figure, the probe is limited to a single diagnositic band, and that is why the other bands are not visible.
Thus, we conducted a secondary screen of another pooled 6 8 workers on any colonies for which the b allelic protein band was not visible and pronounced on the initial gel.
For control samples without reverse transcription, this band was not visible.
The straight-invisible condition was the same except that the band was not visible.
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