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However, polyubiquitinated bands were not increased in other combinations.
In addition, the Pitx3 antibody detected non-specific or unknown bands at approximately 95 and 145 kDa, and these bands were not increased by SPIE (data not shown).
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In contrast RCAN1 isoform 1 (38 kDa band) [26] expression was not increased by PROK1 treatment (Fig. 3C).
The birthrate is not increasing.
Illegal immigration is not increasing.
However, when the DNA was incubated with increasing concentrations of the protein, additional shifted bands were not formed.
Macroscopic shear bands were not seen.
Other, non-specific bands were not detected.
Samples were re-extracted if ribosomal bands were not visible.
However, these bands were not detected by anti-Myc immunoblotting.
The bands were not expressed in negative control samples.
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