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Before calculation of treatment effects corresponding bands were normalized using the measured amounts of the housekeeping protein GAPDH.
The relative expression levels for the dystrophin bands were normalized using tubulin bands within the same linear range of detection.
Bands were normalized using β-actin antibody.
To compare relative band densities between immunoblots, all bands were normalized using β-actin band densities.
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All of the membranes were analyzed by scanning densitometry and the quantification of bands was normalized using internal standards for each band.
Band densities were normalized using glyceraldehyde‐3‐phosphate dehydrogenase and quantified using a scanning densitometric analysis Image J software (National Institutes of Health, Bethesda, MD).
Images were analyzed by digital fluorescence imager (Storm 860; GE Healthcare Bio-Sciences) and band densities were normalized using actin as total extracts calibrator with ImageQuant TL (GE Healthcare Bio-Sciences).
Data were normalized using quantile normalization.
Gels were analyzed using densitometry and bands were normalized to Actin.
Bands were normalized to β-actin expression which was used as an internal loading control.
Protein bands were quantified using densitometry software (Alpha Innotech, San Leandro, CA), and bands were normalized to the actin band as a loading control.
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