Sentence examples for bands were manually excised from inspiring English sources

Gel bands were manually excised, reduced, alkylated and digested overnight with bovine trypsin (Roche, Basel, Switzerland) as described elsewhere [20].

Protein bands were manually excised and digested in-gel with trypsin using an Investigator Progest robot (Genomic Solutions, Huntingdon, UK).

Silver-stained protein spots or bands were manually excised with a P2D1.5 pen (The Gel Company) from 2D and 1D gels and destained with a 1∶1 working solution of 30 mM potassium ferricyanide and 100 mM sodium thiosulfate.

Coomassie stained bands were manually excised and processed for mass spectrometry as follows.

The gel was stained with Coomassie and H3 bands were manually excised, propionylated and digested with trypsin as described before with minor modifications.

Protein bands were manually excised from the gels, and after trypsin digestion, these were analyzed on a 4800 Plus MALDI TOF/TOF Analyzer (Applied Biosystems, USA).

In brief, bands of interest were manually excised from the gel and washed with deionized water followed by two washes with 100% acetonitrile for 15 and 2 minutes, respectively.

Bands from SDS-PAGE were manually excised, triturated, reduced, S-alkylated and digested over night at 37°C with modified trypsin (Sigma-Aldrich, USA).

Bands and spots of interest were manually excised, destained in ammonium bicarbonate 2.5 mM and acetonitrile 50% (v/v), and acetonitrile dehydrated.

Briefly, the protein bands from the 1-D gel were manually excised from the gel and cut into small pieces (∼0.5 mm3).

Protein bands in the appropriate relative molecular weight range were manually excised, reduced with 5 mM dithiothreitol (DTT) and alkylated with 50 mM iodoacetamide (Bio-Rad Laboratories), digested with trypsin (Promega Corp., Madison, WI) and eluted by diffusion.