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After developing the x-ray film, the polymorphic bands were located by either marking with a pencil or cutting through the developed x-ray film.
Following staining with ethidium bromide, specific DNA bands were located by UV translumination, sliced from the gel, and purified by using Qiaquick spin columns (Qiagen, Valencia, CA).
Bands were located by scanning with a Typhoon imager (GE, Pittsburgh, PA) for fluorescein fluorescence, and excised gel slices were immersed in approximately 500 µl RNase-free H2O in non-stick tubes overnight at 4°C to elute the RNA.
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The areas of new bones between the inner of red fluorescent bands and the lateral of yellow fluorescent bands were located and measured by Scion Image software (version beta 4.0.3).
Differentially expressed bands were located, excised from the gel, amplified by PCR, and cloned in the pT/Adv vector using the AdvanTage PCR cloning kit (Clontech), in accordance with the manufacturer's instructions.
In POC, these bands were located at 920 and 835 cm−1.
All bands are located at ∼72kD, the predicted weight of Perforin-2.
ChIP Peaks were located by MPeak [79].
Next energy band is located lower than 10 eV and formed by the S 3s and As 4s electrons.
The heaviest snow band was located in the eastern suburbs, with another band located well west of D.C.
This band is located at 1577 cm−1, in the spectrum of B-DNA.
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