Dominant bands were excised from the gels and re-amplified, and the fragments were recovered and cloned again.
The significantly different bands were excised from the gels and analyzed by Q-TOF LC/MS, after trypsin digestion.
The selected bands were excised from the polyacrylamide gel, and the amplified DNA was recovered using Poly-Gel DNA Extraction Kit (OMEGA Bio-Tek, USA).
Bands were excised from the gel and purified using the NucleoSpin® Gel and PCR Clean-up (Macherey Nagel) following manufacturer's recommendations.
The 1.4 kb bands were excised from gels using a sterilized scalpel.
The separated proteins were silver-stained and the bands were excised from the stained gel.
As in DGGE, the bands were excised from the gels, reamplified and sequenced.
Fluorescent bands were excised from the native gel and subjected to SDS-PAGE followed by western blot analysis.
These select bands were excised from the gel and eluted using the protocol described by Long and Azam [42].
The bands were excised from the gel, digested with trypsin, and the tryptic peptides identified by mass spectrometry.
Bands were excised from the silver stained gel and digested with trypsin to release peptides for LC-MS/MS.
