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Active fractions were re-run on a 12% SDS-PAGE [133] and bands were excised for nano-liquid chromatography peptide separation and mass spectrometry.
Protein bands were excised for in-gel tryptic digestion.
The supernatant was separated by SDS PAGE and bands were excised for LC-MS/MS analysis.
Bands were excised for liquid chromatography-mass spectrometry analysis at SickKids Mass Spectrometry facility.
To determine whether the L. plantarum ZJ316 colonized in pigs' guts, most of the single DGGE bands were excised for cloning and sequencing.
In initial experiments, proteins were separated by SDS/10% PAGE and the bands were excised for analysis by LC-MS/MS after in-gel trypsin digestion.
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Reactions were resolved by SDS-PAGE and either the myrLckNT bands were excised from gels and treated for scintillation counting or gels were treated for fluorography and exposed to film.
Selected bands were excised from the DGGE gel for reamplification and comparative sequencing.
Gel slices (S1 S4) containing these two as well other bands were excised from the gel and tested for SHL8 activity after treating the eluted material with trypsin and CPB (Fig. 4F).
The visualized bands were excised from the gel and treated for mass spectrometry fingerprint analysis as following: they were washed 15 min at RT with acetonitril 30%, incubated 35 min at 56°C in 1,4,-dithioerythritol 10 mM, followed by 30 min incubation in iodoacetamid 55 mM at room temperature in the dark.
Protein bands were excised from the gel, using a sterile scalpel, for mass spectroscopy analysis.
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