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Blocked bands were corrected for background and the unknown samples were also corrected by subtracting the fluorescent optical density of a sample that was not induced for protein overexpression.
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The density of each protein band was corrected for background and expressed in arbitrary units.
After background subtraction the optical density (OD) value for each individual protein band was corrected for β-actin loading and normalized to the vehicle control expressed as l.
Densitometry analysis was carried out by using ImageJ64, and intensities of bands were corrected to control lanes for each individual blot.
Cleavage was calculated using the following equation: cleavage = 100 × (CL /[ FL) + (CL ], where FL and CL are the intensities of the respective bands that were corrected for methionine content.
CRM1 band intensities were corrected for differences in GST-NESs band intensities and normalized to wild-type control intensity in each set of mutations.
Gels were scanned and band intensities were corrected for the total intensity of protein bands on the corresponding SDS-PAGE Coomassie Blue stained gels, and then normalized for exposure differences by dividing by the mean band intensity.
The band intensities were corrected for the number of guanines in each sequence so that the intensity values report on the relative counts of RNA molecules within each band.
Where required, band intensities were corrected for unequal loading by reprobing the blots for actin.
As shown in Figure 6(b), densitometry quantitated band intensities were corrected for loading using housekeeping gene HPRT1 as a control and graphed as a ratio of HPRT1.
The Landsat ETM+ bands of 2013 were corrected for the SLC-off (Scan Line Corrector failed) by using image enhancement techniques and nearest-neighbor interpolation.
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